种属

人

别称

SKNDZ

组织来源

大脑

疾病

神经母细胞瘤细胞

传代比例/细胞消化

1:2传代,

完全培养基配置

DMEM培养基 ；10%胎牛血清 ；1%双抗

简介

SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line.

形态

成神经细胞样

生长特征

贴壁生长

STR

Amelogenin X CSF1PO 12 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11

D16S539 9,11 D18S51 15,17 D21S11 29,31 FGA 18,22 Penta D 9,14 Penta E 7,10 TH01 6,9 TPOX 8

vWA 16,18

倍增时间

每周 2 至 3 次

培养条件

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。

冻存条件

冻存液：90%FBS，DMSO 10%,

或使用非程序冻存液：官网货号JY-H040

保藏机构

ATCC; CRL-2149

备注

该细胞贴壁松散，收货如有大块脱落或全部脱落的细胞团，为正常现象，请按照收货注意事项处理。

产品使用

仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。